ECE 6460 - Biophotonics

This course will cover the basics fo physical processes, instrumentation, and methods related to biophotonics. Topics will include interaction of light with molecules (absorption, fluorescence, energy transfer, lifetime, Raman scattering, nonlinear processes); optical probes (common fluorophores, absorbing/scattering labels, Q-dots, Raman labels); optical microscopy and imaging (confocal, two-photon, TIRF, SPR, 4-pr, near-field); single molecule detection methods (FCS, improving signal to background); and sensors and microarrays (reactio kinetics, detection methods, applications). There will be one or two laboratory exercises. Lab sessions will be arranged as necessary.