BLCHM 6430 - Structural Methods

This course provides an integrated approach to the applications of NMR, X-ray crystallography, and mass spectrometry in structural biology. Topics covered include: basic NMR theory, and the application of 2D and 3D NMR methods for the determining protein and RNA structures; methods of macromolecular crystallization and crystal structure determination; methods of accurate mass measurement, peptide and oligonucleotide sequencing, and identification of proteins from analysis of protelytic digests in conjunction with database searching. An introduction to molecular modeling will be presented, which will include force fields, energy minimization, and molecular dynamics simulations of biomolecules.